P9136
Pyruvate Kinase from rabbit muscle
Type III, lyophilized powder, 350-600 units/mg protein
Sinónimos:
ATP:pyruvate 2-O-phosphotransferase, PK
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About This Item
Productos recomendados
origen biológico
rabbit muscle
Nivel de calidad
tpo
Type III
Formulario
lyophilized powder
actividad específica
350-600 units/mg protein
mol peso
237 kDa
actividad extraña
lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01%
temp. de almacenamiento
−20°C
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Descripción general
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.
Aplicación
Pyruvate Kinase from rabbit muscle has been used to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) in Pseudomonas putida cells.
Pyruvate kinase has been used in plant spectrophotometric assays to measure ATP hydrolysis . Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency .
Acciones bioquímicas o fisiológicas
Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase (PK) catalyzes an important process of transferring phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP). This reaction results in the conversion of PEP to pyruvate and adenosine triphosphate (ATP). Pyruvate kinase plays a major role in glycolysis and gluconeogenesis. High levels of pyruvate kinase M2 (PKM2) inhibits cell proliferation and tumor growth. Deficiency of PK in red blood cells (RBC) leads to non-spherocytic hemolytic anaemia. In human erythrocytes, deficiency of PK induces a protective effect against Plasmodium falciparum.
Definición de unidad
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
Nota de análisis
Protein determined by biuret.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Los clientes también vieron
Red cell pyruvate kinase deficiency: molecular and clinical aspects
Zanella A, et al.
British Journal of Haematology, 130(1), 11-25 (2005)
Hormonal control of pyruvate kinase activity and of gluconeogenesis in isolated hepatocytes
Feliu J E, et al.
Proceedings of the National Academy of Sciences of the USA, 73(8), 2762-2766 (1976)
Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system
Lai B, et al.
Biotechnology for Biofuels, 9(1), 39-39 (2016)
[25] Pyruvate kinase isozymes from rat
Methods in Enzymology, 90, 150-165 (1982)
Pyruvate kinase deficiency and malaria
Ayi K, et al.
The New England Journal of Medicine, 358(17), 1805-1810 (2008)
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