TGEN-CNP
Cas9 Nickase Recombinant protein from Streptococcus pyogenes
lyophilized powder
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.51
form
lyophilized powder
Quality Level
packaging
pkg of 250 μg
pkg of 50 μg
shipped in
ambient
storage temp.
−70°C
General description
Recombinant Cas9 proteins (∼160 KD) are ready-to-use reagents for genome engineering experiments.
When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Features and Benefits
- Highly Specific
- Highly Active
- Ready-to-inject/transfect
Packaging
The kit comes in two pack sizes:
- TGEN-CNP-50UG
- TGEN-CNP-250UG
Components
Each kit consists of:
- one vial of Cas9 recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1mL of Nuclease free water with glycerol
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids.
Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Linkage
Use our CRISPR Selection Tool to order gRNA atsigma.com/crisprs
Know more about Cas9 Nickase Recombinant protein
Know more about Cas9 Nickase Recombinant protein
Physical form
Lyophilized Recombinant_Sp_Cas9 protein
Preparation Note
It is recommended that the lyophilized Cas9 protein be resuspended in the nuclease-free water containing 50% glycerol that is provided in the kit to get the desired
concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.
concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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