T9179
Terrific Broth
EZMix powder microbial growth medium
Sinónimos:
TB media
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About This Item
Código UNSPSC:
41106200
NACRES:
NA.85
Productos recomendados
grado
for molecular biology
Nivel de calidad
descripción
TB; quick dissolve, dust-free formulation
esterilidad
non-sterile
Formulario
powder
técnicas
microbiological culture: suitable
aplicaciones
agriculture
food and beverages
microbiology
temp. de almacenamiento
room temp
idoneidad
nonselective for Escherichia coli
nonselective for coliforms
Descripción general
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Aplicación
Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
Terrific broth has been used:
- as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
- to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
- Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)
Características y beneficios
Terrific Broth EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- A budget-friendly alternative to liquid
- Modified formulation (without glycerol)
- Easy scale-up using larger package sizes
Componentes
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
Reconstitución
Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Información legal
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Protocolos
General protocols for growth of competent cells in microbial medium.
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