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L2897

Sigma-Aldrich

Caldo LB con agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Sinónimos:

Agar Luria Bertani, Caldo Lennox

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.85
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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General description

Lennox LB es un medio para crecimiento microbiano muy citado que se utiliza para el cultivo de E. coli. Este caldo microbiano rico en nutrientes contiene péptidos, aminoácidos y carbohidratos en una formulación baja en sales.

Application

El caldo LB con agar (Lennox) se ha utilizado para:

  • la transformación de las células de E. coli competentes[1]
  • [1] para cultivar bacterias Ecc-15[2]
  • incorporar andamios que contienen tejido derivado de células madre pluripotentes inducidas (iPSCs)
  • microscopia electrónica de transmisión(TEM)[3][4] e inmunohistoquímica (IHC)[3]
Es adecuado para el cultivo no selectivo de cepas de E. coli para la clonación, la producción de plásmidos de ADN y la producción de proteínas recombinantes. También es adecuado para el cultivo selectivo cuando se añaden los antibióticos apropiados, entre ellos los que requieren bajas condiciones salinas, como la zeocina®.

Features and Benefits

El polvo Lennox LB con agar proporciona:
  • Una alternativa económica a las placas precargadas
  • Fácil escalado utilizando tamaños de envase mayores
  • Una formulación estándar

Preparation Note

1. Suspenda 35 g en 1 l de agua destilada.
2. Caliente hasta ebullición con agitación para disolver por completo todos los ingredientes.
3. Esterilice en autoclave durante 15 minutos a 121 ºC.
Para preparar el agar Lennox L: Añada 1 g de glucosa y siga las instrucciones de preparación que se acaban de indicar. Para preparar el medio de Enquist y Sternberg: Agregue asépticamente 10 ml de sulfato de magnesio 1 M estéril después de haberlo esterilizado en autoclave.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

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Referencia del producto
Descripción
Precios

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LB Broth (Lennox), Tablet microbial growth medium

L7025

LB Broth with agar (Lennox), Tablet microbial growth medium

L7658

LB Broth (Lennox), EZMix powder microbial growth medium

L7533

LB Broth with agar (Lennox), EZMix powder microbial growth medium

L3022

Caldo LB (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

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CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000419441
0000419441
0000409284
0000407190
0000402283

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Ultrastructural visualization of the Mesenchymal-to-Epithelial Transition during reprogramming of human fibroblasts to induced pluripotent stem cells
Hoffding, MK and Hyttel, P
Current Stem Cell Research & Therapy, 14.0, 39-53 (2015)
Farbod Alimohammadi et al.
Langmuir : the ACS journal of surfaces and colloids, 34(24), 7192-7200 (2018-05-22)
Two-dimensional (2D) nanomaterials have attracted considerable attention in biomedical and environmental applications due to their antimicrobial activity. In the interest of investigating the primary antimicrobial mode-of-action of 2D nanomaterials, we studied the antimicrobial properties of MnO2 and MoS2, toward Gram-positive
Structural analysis of three-dimensional human neural tissue derived from induced pluripotent stem cells
Brooks PT, et al.
Journal of Separation Science, 6.0, 337-337 (2016)
Martin Wegner et al.
Bio-protocol, 10(1), e3472-e3472 (2020-01-05)
Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA
Shun Gen Huang et al.
BMC immunology, 14, 27-27 (2013-06-22)
Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible
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Protocolos

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Questions

1–5 of 5 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. What is the difference among the many LB - Lennox products?

    1 answer

    1. LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix™ powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

      Helpful?

  3. What are the differences among the Luria, Lennox and Miller LB formulations?

    1 answer

    1. LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version).

      Helpful?

  4. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  5. Will adding magnesium to the culture medium increase cell density?

    1 answer

    1. In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator.

      Helpful?

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