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T9069

Sigma-Aldrich

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

Sinónimos:

DNA Topoisomerase I

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About This Item

Comisión internacional de enzimas:
5.99.1.2 (BRENDA, IUBMB)
Número MDL:
MFCD03458765
Código UNSPSC:
12352204
NACRES:
NA.54
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recombinante

expressed in baculovirus

Nivel de calidad

200

Formulario

buffered aqueous glycerol solution

mol peso

100 kDa

concentración

≥2 units/μL

Nº de acceso UniProt

P11387

temp. de almacenamiento

−70°C

Información sobre el gen

human ... TOP1(7150)

Descripción general

Topoisomerase I (TOP1) is mapped to human chromosome 20q12.[1] TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.[2]

Aplicación

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. [3] Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. [4]
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.[5]

Acciones bioquímicas o fisiológicas

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation.[2] TOPI is essential for normal development and transcriptional regulation.[6] Variants in the TOP1 gene is observed in patients with colorectal cancer.[1]
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

Definición de unidad

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

Forma física

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H360D

Consejos de prudencia

P201 - P308 + P313

Clasificaciones de peligro

Repr. 1B

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000418256
0000418256
SLCQ3918
0000389089
0000389089

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Binding and dissociation of human topoisomerase I with hairpin-loop RNAs: implications for the regulation of HIV-1 replication
Takahashi H, Sawa H, Hasegawa H,
Biochemical and Biophysical Research Communications, 297(3), 593-599 (2012)
M Lisby et al.
The Journal of biological chemistry, 276(23), 20220-20227 (2001-04-03)
All eukaryotic forms of DNA topoisomerase I contain an extensive and highly charged N-terminal domain. This domain contains several nuclear localization sequences and is essential for in vivo function of the enzyme. However, so far no direct function of the
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA
Redinbo MR, et al.
Biochemistry, 39(23), 6832-6840 (2000)
Topoisomerase I in human disease pathogenesis and treatments
Li M and Liu Y
Genomics, Proteomics & Bioinformatics, 14(3), 166-171 (2016)
Molecular Networking Reveals Two Distinct Chemotypes in Pyrroloiminoquinone-Producing Tsitsikamma favus Sponges
Kalinski JCJ, et al.
Marine drugs, 17(1), 60-60 (2019)
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Questions

  1. Is there any determined protocol to inactivate the enzyme after the reaction?

    1 answer

    1. Unfortunately, a protocol for inactivation of this enzyme is not available. There are a number of publications regarding the inactivation and inhibition of Topoisomerases. Please review the links below to publications that may offer additional information.
      Heat sensitivity:
      https://pubmed.ncbi.nlm.nih.gov/8188763/
      https://pubmed.ncbi.nlm.nih.gov/8854885/

      Topoisomerase inhibitors:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2707511/

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