Saltar al contenido
Merck

SRP0290

Sigma-Aldrich

Cathepsin F Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

Sinónimos:

CATSF, CLN13

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Comisión internacional de enzimas:
Código UNSPSC:
12352204
NACRES:
NA.54

recombinante

expressed in FreeStyle 293-F cells

Ensayo

≥90% (SDS-PAGE)

Formulario

aqueous solution

actividad específica

≥18 pmol/min-μg

mol peso

53 kDa

técnicas

activity assay: suitable

solubilidad

soluble
water: soluble

Nº de acceso NCBI

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... CTSF(8722)

Descripción general

Research area: CELL SIGNALING

Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 20-end), with C-terminal HIS tag, MW = 53 kDa (more than 58 kDa after post-modification), expressed in FreeStyle 293-F cells.

Aplicación

Active human cathepsin F is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin F has been used in a study to assess its implications for the development of novel immunomodulators. Active human cathepsin F has also been used in a study to investigate microarray expression analysis and identification of serum biomarkers for Niemann-Pick disease.

Acciones bioquímicas o fisiológicas

Cathepsin F (CtsF) are endopeptidases that play a role in lysosomal protein degradation. Beyond its involvement in protein recycling, CtsF has also been associated with immune responses mediated by Toll-like receptors (TLRs) and antigen processing. Additionally, mutation in the cathepsin F gene is related to the development of familial papillary thyroid cancer , type B Kufs disease, defective lysosomal degradation and abnormal autophagy, and Alzheimer′s disease.

Definición de unidad

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Forma física

Formulated in 45 mM sodium acetate, 135 mM NaCl and 10% glycerol.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Información legal

FreeStyle is a trademark of Invitrogen Corp.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Celine V M Cluzeau et al.
Human molecular genetics, 21(16), 3632-3646 (2012-05-24)
Niemann-Pick disease type C (NPC) is a lysosomal storage disorder characterized by liver disease and progressive neurodegeneration. Deficiency of either NPC1 or NPC2 leads to the accumulation of cholesterol and glycosphingolipids in late endosomes and early lysosomes. In order to
Cathepsin F genetic mutation is associated with familial papillary thyroid cancer
Wang Y, et al.
Journal of Separation Science, 364(4), 414-424 (2022)
Cathepsins in the Pathophysiology of Mucopolysaccharidoses: New Perspectives for Therapy
Pasquale VD, et al.
Cells, 9(4), 979-979 (2020)
Salma Teimoori et al.
Parasitology international, 66(4), 443-447 (2016-05-04)
Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. Therefore sensitive and specific
The Crystal Structure of Human Cathepsin F and Its Implications for the Development of Novel Immunomodulators
Somoza JR, Palmer JT, Ho JD.
Journal of Molecular Biology, 322(3), 559-568 (2012)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico