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SAE0091

Sigma-Aldrich

Lisostafina from Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

Sinónimos:

Endopeptidasa glicil-glicina

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About This Item

Número de CAS:
9011-93-2
Comisión internacional de enzimas:
3.4.24.75 (BRENDA, IUBMB)
Número MDL:
MFCD00131556
Código UNSPSC:
12352202
NACRES:
NA.54

Nombre del producto

Lisostafina from Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

origen biológico

microbial (Staphylococcus staphylotyticus)

Formulario

lyophilized powder

actividad específica

≥500 units/mg protein

mol peso

27 kDa

Características

DNA free

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Aplicación

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Acciones bioquímicas o fisiológicas

La lisostafina es una endopeptidasa de cinc con un peso molecular de aproximadamente 25 kDa. Debido a que la lisostafina rompe los enlaces transversales de la poliglicina de la capa de peptidoglucanos de la pared celular de las especies de Staphylococcus , se ha encontrado útil para la lisis celular y también como potencial tratamiento antimicrobiano.
pH de actividad óptimo: ~7,5
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.

Definición de unidad

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Forma física

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000395833
0000314989
0000395833
0000220707
0000182997

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Kojiro Tsujihana et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2116027119-e2116027119 (2022-06-16)
The epidermis is the outermost layer of the skin and the body's primary barrier to external pathogens; however, the early epidermal immune response remains to be mechanistically understood. We show that the chemokine CXCL14, produced by epidermal keratinocytes, exhibits robust
Ronald M Galiwango et al.
Microbiome, 10(1), 7-7 (2022-01-20)
Coronal sulcus (CS) anaerobe abundance and IL-8 levels are linked to HIV acquisition, and are dramatically reduced after penile circumcision (PC). The distal urethra may be the site of some HIV acquisition before PC, and presumably most acquisition post PC. We
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
Caroline Meyer Olesen et al.
Microorganisms, 9(7) (2021-08-08)
Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin
Astrid Haaskjold Lossius et al.
Dermatology (Basel, Switzerland), 238(1), 109-120 (2021-04-23)
The pathophysiology in atopic dermatitis (AD) is not fully understood, but immune dysfunction, skin barrier defects, and alterations of the skin microbiota are thought to play important roles. AD skin is frequently colonized with Staphylococcus aureus (S. aureus) and microbial
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