S4195

β-Secretase human

recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), extracellular domain, ≥10,000 units/mg protein, ≥90% (SDS-PAGE), buffered aqueous solution

• Sinónimos: Asp-2, BACE1, Memapsin-2
• Número MDL: MFCD03702801
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• recombinante: expressed in HEK 293 cells (C-terminal FLAG tagged)
• Nivel de calidad: 200
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: buffered aqueous solution
• actividad específica: ≥10,000 units/mg protein
• Nº de acceso UniProt: P56817
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... BACE1(23621)

Descripción

Aplicación

Human β-secretase or BACE1 has been used to study the binding reaction between amyloid precursor protein and β-secretase. It has also been used in BACE1 assay.[1][2]

Acciones bioquímicas o fisiológicas

BACE1 (β−Secretase or β−Site APP-Cleaving Enzyme) is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s, therefore BACE1 is a target for inhibitor drug discovery.

BACE1 (β-site APP-cleaving enzyme 1) is a transmembrane aspartyl protease responsible for cleaving the amyloid precursor protein (APP). BACE1-mediated cleavage of amyloid precursor protein (APP) is the first step during the generation of pathogenic amyloid-β peptides, hence making it the major drug target for Alzheimer′s disease (AD). Increased levels of enzymatic activity of BACE1 was found in cerebrospinal fluid from patients having both mild cognitive impairment (MCI) and AD, thereby suggesting the importance of BACE1 enzymatic activity in conversion of MCI to AD.[3][4]

Transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide.

Definición de unidad

One unit will hydrolyze 1.0 picomole of 7-methoxycoumarin-4-acetyl-[Asn⁶⁷⁰, Leu⁶⁷¹]-Amyloid β/A4 Precursor Protein 770 Fragment 667-676-(2,4-dinitrophenyl)-Lys-Arg-Arg amide substrate per 1 minute at pH 4.5 at 37 °C.

Forma física

Solution in 20 mM Hepes, pH 7.4, 125 mM NaCl.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)