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Merck

S4195

Sigma-Aldrich

β-Secretase human

recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), extracellular domain, ≥10,000 units/mg protein, ≥90% (SDS-PAGE), buffered aqueous solution

Sinónimos:

Asp-2, BACE1, Memapsin-2

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About This Item

Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32

recombinante

expressed in HEK 293 cells (C-terminal FLAG tagged)

Nivel de calidad

Ensayo

≥90% (SDS-PAGE)

Formulario

buffered aqueous solution

actividad específica

≥10,000 units/mg protein

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Información sobre el gen

human ... BACE1(23621)

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Aplicación

Human β-secretase or BACE1 has been used to study the binding reaction between amyloid precursor protein and β-secretase. It has also been used in BACE1 assay.

Acciones bioquímicas o fisiológicas

BACE1 (β−Secretase or β−Site APP-Cleaving Enzyme) is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s, therefore BACE1 is a target for inhibitor drug discovery.
BACE1 (β-site APP-cleaving enzyme 1) is a transmembrane aspartyl protease responsible for cleaving the amyloid precursor protein (APP). BACE1-mediated cleavage of amyloid precursor protein (APP) is the first step during the generation of pathogenic amyloid-β peptides, hence making it the major drug target for Alzheimer′s disease (AD). Increased levels of enzymatic activity of BACE1 was found in cerebrospinal fluid from patients having both mild cognitive impairment (MCI) and AD, thereby suggesting the importance of BACE1 enzymatic activity in conversion of MCI to AD.
Transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide.

Definición de unidad

One unit will hydrolyze 1.0 picomole of 7-methoxycoumarin-4-acetyl-[Asn670, Leu671]-Amyloid β/A4 Precursor Protein 770 Fragment 667-676-(2,4-dinitrophenyl)-Lys-Arg-Arg amide substrate per 1 minute at pH 4.5 at 37 °C.

Forma física

Solution in 20 mM Hepes, pH 7.4, 125 mM NaCl.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Visite la Librería de documentos

Dennis J Selkoe
Nature cell biology, 6(11), 1054-1061 (2004-11-02)
The salutary intersection of fundamental cell biology with the study of disease is well illustrated by the emerging elucidation of neurodegenerative disorders. Novel mechanisms in cell biology have been uncovered through disease-orientated research; for example, the discovery of presenilin as
cceleration of deposition of peptide on ultrasonically formed nucleus studied by wireless quartz-crystal-microbalance biosenso
Hirotsugu Ogi
Biosensors, 40(1) (2013)
Platelet-Derived Secreted Amyloid-Precursor Protein-? as a Marker for Diagnosing Alzheimer?s Disease
Current neurovascular research (2013)
Roland Nicsanu et al.
Journal of Alzheimer's disease : JAD, 87(1), 433-441 (2022-03-12)
Beta-site APP cleaving enzyme 1 (BACE1) is the rate-limiting enzyme in amyloid-β (Aβ) plaques formation. BACE1 activity is increased in brains of patients with Alzheimer's disease (AD) and mild cognitive impairment (MCI) and plasma levels of BACE1 appears to reflect
Carlo Cervellati et al.
GeroScience, 42(1), 159-167 (2019-11-21)
Beta-secretase (BACE1) is a key enzyme in the formation of amyloid-β; its activity/concentration is increased in brain and cerebrospinal fluid of patients with late-onset Alzheimer's disease (LOAD). Since BACE1 was found also in blood, we evaluated its potential as peripheral

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