S3917
Single-strand Binding Protein from Escherichia coli
>95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity
Sinónimos:
SSB
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About This Item
Productos recomendados
grade
for molecular biology
Quality Level
assay
>95% (SDS-PAGE)
form
liquid
color
colorless
foreign activity
DNase and RNase, none detected
shipped in
wet ice
storage temp.
−20°C
General description
Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.
Application
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.
Biochem/physiol Actions
Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificados de análisis (COA)
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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 87, 5-5 (1989)
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.
Q Chou
Nucleic acids research, 20(16), 4371-4371 (1992-08-25)
Binding Specificity of E. coli SSB protein for the ? subunit of DNA pol III Holoenzyme and PriA helicase
Alexander GK, et al.
Biochemistry, 49(17), 3555-3556 (2010)
J Kieleczawa et al.
Science (New York, N.Y.), 258(5089), 1787-1791 (1992-12-11)
When template DNA is saturated with a single-stranded DNA binding protein (SSB), strings of three or four contiguous hexanucleotides (hexamers) can cooperate through base-stacking interactions to prime DNA synthesis specifically from the 3' end of the string. Under the same
The Integron Integrase Efficiently Prevents the Melting Effect of Escherichia coli Single-Stranded DNA-Binding Protein on Folded attC Sites
Loot C, et al.
Journal of Bacteriology, 196(4), 762-771 (2014)
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