MAK133
ADP Assay Kit
sufficient for 100 assays (bioluminescent)
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Productos recomendados
uso
sufficient for 100 assays (bioluminescent)
método de detección
chemiluminescent
enfermedades relevantes
hematological disorder; cancer
temp. de almacenamiento
−20°C
Descripción general
Adenosine diphosphate (ADP) is a nucleoside that plays a critical role in energy transfer reactions. ADP is produced from adenosine triphosphate via the action of ATPases. ADP also plays a critical role in platelet function. ADP, stored in plate-dense granules, is released upon platelet activation where it acts on purinergic receptors to mediate intracellular signaling and platelet aggregation.
The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the Substrate (D-luciferin) to produce light. The light intensity is a direct measure of the intracellular ATP concentration and is stable over several minutes.
In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.
The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the Substrate (D-luciferin) to produce light. The light intensity is a direct measure of the intracellular ATP concentration and is stable over several minutes.
In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.
Idoneidad
Suitable for the detection of ADP in cells, tissue and other biological samples.
Principio
The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the substrate (D-luciferin) to produce light. The light intensity is a direct measure of the intracellular ATP concentration and is stable over several minutes.
Luciferase ATP + D-Luciferin + O2 → oxyluciferin + AMP + PPi + CO2 + light
In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.
Luciferase ATP + D-Luciferin + O2 → oxyluciferin + AMP + PPi + CO2 + light
In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Skin Sens. 1
Código de clase de almacenamiento
10 - Combustible liquids
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