H8889
Histopaque®-1077 Hybri-Max™
liquid, sterile-filtered, BioReagent, suitable for hybridoma
Sinónimos:
Histopaque 1077
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About This Item
Código UNSPSC:
12352201
NACRES:
NA.75
Productos recomendados
esterilidad
sterile-filtered
Nivel de calidad
Línea del producto
BioReagent
Formulario
liquid
técnicas
cell culture | hybridoma: suitable
single cell analysis: suitable
impurezas
endotoxin, tested
densidad
1.077 at 25 °C (relative density)(lit.)
temp. de almacenamiento
2-8°C
Descripción general
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Aplicación
Histopaque®-1077 Hybri-Max™ has been used to isolate:
- peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
- immune cells (lymphocytes) from peripheral blood
- PBMCs from blood
Información legal
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Protocolos
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
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