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H3917

Sigma-Aldrich

Heparinase I and III Blend from Flavobacterium heparinum

lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)

Sinónimos:

Heparinase I and Heparinase III blend

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About This Item

Comisión internacional de enzimas:
4.2.2.7 (BRENDA, IUBMB)
Código UNSPSC:
12352204
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

bacterial (Flavobacterium heparinum)

Nivel de calidad

200

conjugado

conjugate (Glucosaminoglycan)

Formulario

lyophilized powder

actividad específica

≥200 units/mg protein

concentración

≥200 unit/mg protein (enzyme + BSA)

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin.[1]

Aplicación

Heparinase I and III Blend from Flavobacterium heparinum has been used in:
  • the digestion of heparan sulfate from ovine vitreous[2]
  • human embryonic kidney cells[3]
  • glycosaminoglycans from arterial tissues[4]
  • P0 retinae digestion[5]

Acciones bioquímicas o fisiológicas

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization.[6][7][8] It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.[9]

Envase

Sold on the basis of Heparinase I units

Definición de unidad

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I.

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III.

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.

Otras notas

Enzyme Commission Numbers: 4.2.2.7 Hep I and 4.2.2.8 Hep III

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000312746
0000312745
0000312744
0000312746
0000312745

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Visite la Librería de documentos

R Sasisekharan et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(4), 1524-1528 (1994-02-15)
Neovascularization is associated with the regulation of tissue development, wound healing, and tumor metastasis. A number of studies have focused on the role of heparin-like molecules in neovascularization; however, little is known about the role of heparin-degrading enzymes in neovascularization.
Glycosaminoglycans contribute to extracellular matrix fiber recruitment and arterial wall mechanics
Mattson JM, et al.
Biomechanics and Modeling in Mechanobiology, 16(1), 213-225 (2017)
Retinal proteoglycans act as cellular receptors for basement membrane assembly to control astrocyte migration and angiogenesis
Tao C and Zhang X
Cell Reports, 17(7), 1832-1844 (2016)
Diffusion regulation in the vitreous humor
Kasdorf B, et al.
Biophysical Journal, 109(10), 2171-2181 (2015)
IL-2 inducible kinase ITK is critical for HIV-1 infection of Jurkat T-cells
Hain A, et al.
Scientific reports, 8(1), 3217-3217 (2018)
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