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H2508

Sigma-Aldrich

Hippuryl-Arg

carboxypeptidase substrate, chromogenic, ≥98% (TLC), powder

Sinónimos:

N-Benzoyl-Gly-Arg

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About This Item

Fórmula empírica (notación de Hill):
C15H21N5O4
Número de CAS:
744-46-7
Peso molecular:
335.36
EC Number:
212-015-1
MDL number:
MFCD00055706
UNSPSC Code:
12352204
PubChem Substance ID:
24895502
NACRES:
NA.32

product name

Hippuryl-Arg, carboxypeptidase substrate

assay

≥98% (TLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

NC(=N)NCCC[C@H](NC(=O)CNC(=O)c1ccccc1)C(O)=O

InChI

1S/C15H21N5O4/c16-15(17)18-8-4-7-11(14(23)24)20-12(21)9-19-13(22)10-5-2-1-3-6-10/h1-3,5-6,11H,4,7-9H2,(H,19,22)(H,20,21)(H,23,24)(H4,16,17,18)/t11-/m0/s1

InChI key

GFLCPYUSPYXNBV-NSHDSACASA-N

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Application

Hippuryl-Arg has been used as a substrate for:
  • aminopeptidase from Trogoderma granarium
  • exopeptidase from Bactrocera oleae Gmelin, midgut of Plagiodera versicolora
  • carboxypeptidase from Pichia pastoris

Biochem/physiol Actions

Hippuryl-Arg is a synthetic substrate for carboxypeptidase B, and aminopeptidase. The enzyme carboxypeptidase acts on C terminal of arginine and lysine.

Substrates

Substrate for carboxypeptidase B and carboxypeptidase N.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Proteolytic activity in Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae): Characterization of digestive proteases and effect of host plants
Zibaee A and Hajizadeh J
Journal of Asia-Pacific Entomology, 16(3), 329-334 (2013)
Hidefumi Komura et al.
Microbiology and immunology, 46(2), 115-117 (2002-04-10)
Carboxypeptidases (CP) in plasma and sera serve as regulators of anaphylatoxins such as C3a and C5a. The activity of CP can be measured by determining hippuric acid after cleavage of the small synthetic substrate hippuryl-L-arginine. Although a colorimetric assay is
Expression, purification and characterization of porcine pancreatic Carboxypeptidase B from Pichia pastoris for the conversion of recombinant human insulin
Mansur M, et al.
Enzyme and Microbial Technology, 40(3), 476-480 (2007)
S Appelros et al.
Biological chemistry, 379(7), 893-898 (1998-08-15)
Recent developments in the treatment of acute pancreatitis have focused on the importance of early determination of the severity of an attack. Measuring levels of activation peptides from pancreatic proenzymes seems to be one way to predict severity. Levels of
Yu-Hong Liu et al.
FEBS letters, 580(22), 5137-5142 (2006-09-12)
This work reports the use of electrospray mass spectrometry for studying the conformational dynamics of enzymes by amide hydrogen/deuterium exchange (HDX) measurements. A rapid-mixing quench-flow approach allows comparisons to be made between the HDX kinetics of free enzymes with those
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