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GE29-0510-21

HisTrap™ High Performance

Cytiva 29-0510-21, pack of 1 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

envase

pack of 1 mL

fabricante / nombre comercial

Cytiva 29-0510-21

disponibilidad

not available in North America

Parámetros

flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

lecho tamaño

7 mm × 25 mm

lecho volumen

1 mL

columna D.I.

7 mm

Matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning

2-14(Ni2+-stripped medium.)

Intervalo de trabajo

3-12(Ni2+-stripped medium)

capacidad

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
 ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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Descripción general

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Aplicación

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

Características y beneficios

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Información legal

HisTrap is a trademark of Cytiva

Pictogramas

Environment
GHS09

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ver todo

Protocolos

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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