GE28-4082-55
HiTrap® MabSelect™
Cytiva 28-4082-55, pack of 1 × 5 mL
About This Item
Productos recomendados
ligand
recombinant protein A (E. coli)
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 28-4082-55
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12
working range
3-10
capacity
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
Application
Features and Benefits
- HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
Storage and Stability
Analysis Note
Other Notes
Legal Information
related product
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Artículos
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocolos
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico