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GE17-5160-01

HiTrap® SP XL

Cytiva 17-5160-01, pack of 5 × 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5160-01

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

General description

HiTrap® SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Application

Q Sepharose XL and SP Sepharose XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Features and Benefits

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

related product

Referencia del producto
Descripción
Precios

pictograms

Flame

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

This page covers how and when to use Sepharose XL media for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico