GE17-5072-01
Q Sepharose™ Xl
Cytiva 17-5072-01, pack of 300 mL
About This Item
Productos recomendados
ligand
quaternary amine
descripción
Ion Exchanger Type (value)
envase
pack of 300 mL
fabricante / nombre comercial
Cytiva 17-5072-01
Matriz
6% cross-linked agarose with dextran surface extenders
tamaño de partícula
45-165 μm
diámetro promedio
90 μm
cleaning in place
2-14
Intervalo de trabajo
2-12
idoneidad
suitable for bioprocess medium
Categorías relacionadas
Descripción general
Q Sepharose™ XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose™ Fast Flow media. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
With its high loading capacities and excellent physical and chemical stability, Q Sepharose™ XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess media range, Q Sepharose™ XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
Características y beneficios
- High loading capacities give more productive capture from clarified feedstocks.
- Strong anion exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Almacenamiento y estabilidad
Nota de análisis
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
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Artículos
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocolos
This page covers how and when to use Sepharose XL media for purification of proteins.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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