GE17-0780-01
Protein A-Sepharose™ CL-4B
Cytiva 17-0780-01, pack of 1.5 g
Sinónimos:
Protein A Sepharose, Sepharose CL-4B
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About This Item
Código UNSPSC:
41106500
NACRES:
NA.56
Productos recomendados
ligand
native protein A
envase
pack of 1.5 g
fabricante / nombre comercial
Cytiva 17-0780-01
condiciones de almacenamiento
(20% Ehtanol)
Matriz
4% cross-linked agarose
tamaño de partícula
45-165 μm
diámetro promedio
90 μm
cleaning
2-10
Intervalo de trabajo
3-9
capacidad
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
temp. de almacenamiento
2-8°C
Categorías relacionadas
Descripción general
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Características y beneficios
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Almacenamiento y estabilidad
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Nota de análisis
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Información legal
Sepharose is a trademark of Cytiva
Código de clase de almacenamiento
13 - Non Combustible Solids
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