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Merck

G6019

Sigma-Aldrich

Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes

lyophilized powder, 50-150 units/mg protein

Sinónimos:

D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

Formulario

lyophilized powder

Nivel de calidad

actividad específica

50-150 units/mg protein

composición

Protein, 40-70% biuret

Nº de acceso UniProt

actividad extraña

3-Phosphoglyceric phosphokinase ≤2%

temp. de almacenamiento

−20°C

Información sobre el gen

human ... GAPDH(2597)

Acciones bioquímicas o fisiológicas

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Definición de unidad

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Forma física

Lyophilized powder containing sodium citrate buffer salts

Inhibidor

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Tina C Albershardt et al.
Journal of immunological methods, 384(1-2), 196-199 (2012-08-14)
Normalization to a reference gene is the method of choice for quantitative PCR analysis. The stability of reference genes is critical for accurate gene expression analysis, as significant variations in reference gene expression can alter experimental results and conclusions. In
Yuki Ikeda et al.
Archives of biochemistry and biophysics, 528(2), 141-147 (2012-09-25)
Cyclooxygenase (COX)-2 is an inducible inflammatory protein whose expression is partially regulated at the post-transcriptional level. We investigated whether glyceraldehyde-3-phosphate dehydrogenase (GAPDH) binds to the AU-rich element (ARE) of COX-2 mRNA for its degradation. Knockdown of GAPDH in hepa1c1c7 cells
Irina A Sevostyanova et al.
Biochemical and biophysical research communications, 427(3), 649-653 (2012-10-03)
Sperm-specific glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDS) is normally expressed only in sperms, but not in somatic tissues. Analysis of the expression of GAPDS mRNA in different cancer cell lines shows that the content of GAPDS mRNA is enhanced in some
Chiaki Inadomi et al.
Biochemical and biophysical research communications, 425(3), 656-661 (2012-08-01)
There is increasing evidence demonstrating that glutaredoxin 1 (GRX1), a cytosolic enzyme responsible for the catalysis of protein deglutathionylation, plays distinct roles in inflammation and apoptosis by inducing changes in the cellular redox system. In this study, we investigated whether
Anne Gürtler et al.
Analytical biochemistry, 433(2), 105-111 (2012-10-23)
Western blots are used to specifically measure the relative quantities of proteins of interest in complex biological samples. Quantitative measurements can be subject to error due to process inconsistencies such as uneven protein transfer to the membrane. These non-sample-related variations

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