Saltar al contenido
Merck
Todas las fotos(5)

Key Documents

View All Documentation

G4510

Millipore

Glutathione−Agarose

lyophilized powder

Sinónimos:

Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(5)

About This Item

MDL number:
MFCD00131200
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

analyte chemical class(es)

proteins (GST)

extent of labeling

≥10 μmol per mL gel

technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

matrix

4% cross-linked beaded agarose

matrix activation

epoxy

matrix attachment

sulfur

matrix spacer

12 atoms (10 carbon)

storage temp.

−20°C

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.

Application

Glutathione-Agarose has been used:
  • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
  • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
  • in affinity purification of the fusion protein

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Biochem/physiol Actions

Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.

Features and Benefits

  • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
  • Has a shelf life of two years if stored properly desiccated at -20 °C.
  • Binding capacity: 5-10 mg glutathione S-transferase per mL resin

Quantity

One g powder swells to ≥10 ml of gel

Physical form

Lyophilized powder stabilized with lactose

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Wenrong Tan et al.
PLoS genetics, 14(4), e1007336-e1007336 (2018-04-17)
Drought is a major threat to plant growth and crop productivity. The phytohormone abscisic acid (ABA) plays a critical role in plant response to drought stress. Although ABA signaling-mediated drought tolerance has been widely investigated in Arabidopsis thaliana, the feedback
Samir Sayed et al.
Diabetes, 58(6), 1419-1427 (2009-04-02)
Heterozygous activating mutations of glucokinase have been reported to cause hypoglycemia attributable to hyperinsulinism in a limited number of families. We report three children with de novo glucokinase hyperinsulinism mutations who displayed a spectrum of clinical phenotypes corresponding to marked
Pablo C Echeverría et al.
Molecular and cellular biology, 29(17), 4788-4797 (2009-07-08)
Glucocorticoid receptor (GR) is cytoplasmic in the absence of ligand and localizes to the nucleus after steroid binding. Previous evidence demonstrated that the hsp90-based heterocomplex bound to GR is required for the efficient retrotransport of the receptor to the nuclear
Hui Li et al.
Nature communications, 9(1), 2573-2573 (2018-07-04)
The dynamic maintenance of polar domains in the plasma membrane (PM) is critical for many fundamental processes, e.g., polar cell growth and growth guidance but remains poorly characterized. Rapid tip growth of Arabidopsis pollen tubes requires dynamic distribution of active
Magdalena M Grabowska et al.
Molecular endocrinology (Baltimore, Md.), 28(6), 949-964 (2014-05-08)
Androgen receptor (AR) action throughout prostate development and in maintenance of the prostatic epithelium is partly controlled by interactions between AR and forkhead box (FOX) transcription factors, particularly FOXA1. We sought to identity additional FOXA1 binding partners that may mediate
Ver todos los artículos relacionados

Contenido relacionado

Precipitación pull-down de proteínas

Análisis, reactivos y protocolos de precipitación pull-down para investigar las interacciones interproteicas in vitro utilizando métodos de atenuación de precipitación por afinidad o GST, purificación por afinidad en tándem (TAP) y coinmunoprecipitación.

Precipitación pull-down de proteínas

Análisis, reactivos y protocolos de precipitación pull-down para investigar las interacciones interproteicas in vitro utilizando métodos de atenuación de precipitación por afinidad o GST, purificación por afinidad en tándem (TAP) y coinmunoprecipitación.

Precipitación pull-down de proteínas

Análisis, reactivos y protocolos de precipitación pull-down para investigar las interacciones interproteicas in vitro utilizando métodos de atenuación de precipitación por afinidad o GST, purificación por afinidad en tándem (TAP) y coinmunoprecipitación.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico