14-864
PAK-1 PBD Protein (agarose free), 300 µg
For use in Affinity Binding Assays, ELISA & G-Protein Assays.
Sinónimos:
p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32
Productos recomendados
biological source
human
Quality Level
mol wt
Mw 21 kDa
manufacturer/tradename
Upstate®
concentration
>50% (Coomassie blue staining, SDS-PAGE)
2 mg/mL
technique(s)
ELISA: suitable
affinity binding assay: suitable (G-protein)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
General description
Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
Quality
Routinely evaluated by G-protein activity assay
Physical form
300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
Storage and Stability
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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