G2N10
GenElute™ Plant Genomic DNA Miniprep Kit
sufficient for 10 purifications
Sinónimos:
Plant Genomic DNA Miniprep, Gen Elute
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About This Item
Código UNSPSC:
41105501
NACRES:
NA.55
Productos recomendados
uso
sufficient for 10 purifications
características de los productos alternativos más sostenibles
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
categoría alternativa más sostenible
temp. de almacenamiento
15-25°C
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Descripción general
The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
The GenElute Plant Genomic DNA Purification Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of plant species. This kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Aplicación
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
- in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
- in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
- in genomic DNA isolation from leaves
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Características y beneficios
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
Principio
Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
Otras notas
For additional information, please see www.sigma-aldrich.com/genomicdna.
Información legal
GenElute is a trademark of Sigma-Aldrich Co. LLC
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- C2112Column Preparation SolutionSDS
Opcional
Producto relacionado
Palabra de señalización
Danger
Frases de peligro
Clasificaciones de peligro
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Riesgos supl.
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Protocolos
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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