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Merck

R4642

Sigma-Aldrich

Ribonucleasa A from bovine pancreas

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Sinónimos:

ARNasa, ARNasa A, Ribonucleasa pancreática, Ribonucleato 3′-pirimidinooligonucleotidohidrolasa

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.53
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biological source

bovine pancreas

Quality Level

grade

for molecular biology

form

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

mol wt

13.7 kDa
~13,700

concentration

20-40 mg/mL

suitability

suitable for

foreign activity

Endonuclease and exonuclease, none detected
NICKase and DNase, none detected

storage temp.

−20°C

SMILES string

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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General description

La ARNasa, ribonucleasa A, es una endorribonucleasa que escinde los enlaces fosfodiéster del ARN monocatenario después de nucleótidos de pirimidina. Ataca el extremo 3′ fosfato (por ejemplo, el pG-pG-pC-pA-pG será escindido para dar pG-pG-pCp y A-pG). La mayor actividad la exhibe con ARN monocatenario. La ARNasa A es un polipéptido monocatenario que contiene cuatro puentes bisulfuro. Al contrario que la ARNasa B, no es una glucoproteína. Las ribonucleasas no hidrolizan el ADN porque el ADN carece de los grupos 2′-OH esenciales para la formación de los intermediarios cíclicos. La ARNasa A también puede hidrolizar el ARN de las muestras proteicas. La ARNasa A puede ser inhibida por alquilación de la His12 o la His119 y activada por sales de potasio y de sodio. La RNAasa se inhibe en presencia de iones de los metales pesados. La ARNasa también es inhibida competitivamente por el ADN.
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

Application

  • La ribonucleasa A se utiliza para eliminar el ARN de las preparaciones de ADN plasmídico y ADN genómico, y de las muestras de proteínas.
  • La ARNasa se utiliza también en el análisis de la secuencia del ARN y en los análisis de protección.
  • La RNasa A se ha utilizado como herramienta para el diseño de medicamentos asistido por ordenador.
  • La ARNasa A contribuye al análisis de las secuencias de ARN.
  • La ARNasa A hidroliza el ARN contenido en las muestras proteicas.
  • La ARNasa A apoya la purificación del ADN.
Suitable for:
  • RNase protection assays
  • Removal of unspecifically bound RNA
  • Analysis of RNA sequences
  • Hydrolysis of RNA contained in protein samples
  • Plasmid DNA purification

Features and Benefits

Nuestra muy estable ribonucleasa A, ARNasa A, es idónea para la eliminación del ARN, la secuenciación del ARN y la purificación del ADN.

Components

RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).

Unit Definition

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.

Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

Analysis Note

Proteína determinada mediante E.

Other Notes

Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.

Application

Referencia del producto
Descripción
Precios

Inhibitor

Referencia del producto
Descripción
Precios

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificados de análisis (COA)

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Christopher P Selby et al.
The Journal of biological chemistry, 295(50), 17374-17380 (2020-10-23)
In nucleotide excision repair, bulky DNA lesions such as UV-induced cyclobutane pyrimidine dimers are removed from the genome by concerted dual incisions bracketing the lesion, followed by gap filling and ligation. So far, two dual-incision patterns have been discovered: the
Wentao Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6752-6757 (2017-06-14)
Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, is the major cause of lung cancer. BaP forms covalent DNA adducts after metabolic activation and induces mutations. We have developed a method for capturing oligonucleotides carrying bulky base adducts, including UV-induced cyclobutane pyrimidine
R Perdigão-Henriques et al.
Oncogene, 35(2), 158-172 (2015-03-24)
The miR-200 family promotes the epithelial state by suppressing the Zeb1/Zeb2 epithelial gene transcriptional repressors. To identify other miR-200-regulated genes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer cells. In all, 520 mRNAs were significantly enriched
Alessandro Ori et al.
Genome biology, 17, 47-47 (2016-03-16)
Recent large-scale studies revealed cell-type specific proteomes. However, protein complexes, the basic functional modules of a cell, have been so far mostly considered as static entities with well-defined structures. The co-expression of their members has not been systematically charted at
Netta Mäkinen et al.
PLoS genetics, 12(2), e1005850-e1005850 (2016-02-20)
Uterine leiomyosarcomas (ULMSs) are aggressive smooth muscle tumors associated with poor clinical outcome. Despite previous cytogenetic and molecular studies, their molecular background has remained elusive. To examine somatic variation in ULMS, we performed exome sequencing on 19 tumors. Altogether, 43

Artículos

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Protocolos

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

Ver todo

Chromatograms

application for HPLC

Questions

1–6 of 6 Questions  
  1. What is the actual concentration of 10MG R4642, Ribonuclease A in terms of mg/ml?

    1 answer
    1. The mg/mL concentration of this product is lot dependent and reported in the product Certificate of Analysis. The specification for the concentration is 20 - 40 mg/mL. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/r4642#product-documentation

      Please see the link below to review additional information is the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/984/r4642dat.pdf

      Helpful?

  2. Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?

    1 answer
    1. The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.

      Helpful?

  3. Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution.  What does the solution contain?

    1 answer
    1. The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?

    1 answer
    1. This product is stable for at least 2 years when stored properly at -20 °C.

      Helpful?

  6. When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?

    1 answer
    1. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.

      Helpful?

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