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Merck

E1161

Sigma-Aldrich

EDTA Buffer, pH 8.5, 10×, Antigen Retriever

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About This Item

UNSPSC Code:
12352107
NACRES:
NA.47

Application

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8.5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualize antigens by immunohistochemical analysis.

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Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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