You can use MTT to assess the intactness of isolated mitochondria. For this purpose, the CT01-5 Sigma-Aldrich MTT Reagent A is suitable. Additionally, the following publication may be of interest as a protocol reference: PLoS One. 2015 Jul 29;10(7):e0134139. This publication discusses the prevention of cisplatin-induced acute kidney injury and can provide valuable insights.
CS0390
Mitochondria Staining Kit
1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
Sinónimos:
JC-1 dye
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352207
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nivel de calidad
uso
kit sufficient for 200 tests (of 1 mL cell suspensions)
kit sufficient for 40 tests (of 5 mL cell suspensions)
envase
pkg of 1 kit
condiciones de almacenamiento
dry at room temperature
técnicas
flow cytometry: suitable
protein staining: suitable
fluorescencia
λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)
aplicaciones
cell analysis
detection
método de detección
fluorometric
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.
Aplicación
The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.
Características y beneficios
Kit offers:
- A fast, simple, and convenient method for staining and assaying mitochondria intactness
- Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
- The shift in fluorescence is clearly detectable in both green and red channels
- Suitable for adherent cells as well as cells in suspension
- Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
- The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
Solo componentes del kit
Referencia del producto
Descripción
- DMSO 1 mL
- JC Staining Buffer 5X 120 mL
- JC-1 1 mg
- Valinomycin Ready Made .1 mL
Código de clase de almacenamiento
10 - Combustible liquids
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Robert L Elliott et al.
PloS one, 14(4), e0214586-e0214586 (2019-04-02)
Many cells are cultured in media that contains an antibiotic to prevent bacterial contamination. Mycoplasma and other bacterial contamination is a serious problem for those involved in cell culture. Antibiotics in the media helps prevent this contamination and make life
Whasun Lim et al.
Biology of reproduction, 95(4), 82-82 (2016-09-02)
Luteolin is a natural compound known for its anticancer effects on various human cancers by regulating signal transduction cascades. However, the effects of luteolin on human placental choriocarcinoma are not known. Results of the present study revealed that luteolin decreased
A Cossarizza et al.
Biochemical and biophysical research communications, 197(1), 40-45 (1993-11-30)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
Maria C Zanellati et al.
Frontiers in genetics, 6, 78-78 (2015-03-31)
Mutations in PARK2, encoding Parkin, cause an autosomal recessive form of juvenile Parkinson Disease (JPD). The aim of the present study was to investigate the impact of PARK2 mutations on mitochondrial function and morphology in human skin fibroblasts. We analyzed
Sunwoo Park et al.
Journal of cellular biochemistry, 119(9), 7377-7387 (2018-05-16)
Formononetin is an isoflavone that is extracted from red clovers or soy. It has anti-oxidant, anti-proliferative, and anti-tumor effects against cells in various diseases. Several cohort studies have indicated that phytoestrogen intake, including formononetin, could reduce the risk of various
Artículos
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
-
Do you know which product is suitable for evaluating the intactness of isolated mitochondria?
1 answer-
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
