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Merck
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CELLMM2

Millipore

FLAG® M Purification Kit

For Mammalian expression systems.

Sinónimos:

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

technique(s)

protein extraction: suitable

shipped in

wet ice

storage temp.

−20°C

Application

The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Packaging

Sufficient for 3-5 uses of 1-ml affinity purification column.

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDS
  • SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDS
  • A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDS
  • C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDS

Storage Class

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Takeshi Into et al.
Molecular and cellular biology, 28(4), 1338-1347 (2007-12-19)
Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control
M D Mostaqul Huq et al.
Molecular & cellular proteomics : MCP, 6(4), 677-688 (2007-01-09)
Retinoic acid receptors (RARs) belong to the nuclear receptor superfamily. The mechanism of ligand-dependent activation of RARs is well known. The effect of protein phosphorylation on the activity of RARs has also been demonstrated. However, it is unclear whether other
Mayumi Kitagawa et al.
Cell reports, 7(1), 166-179 (2014-03-25)
The chromosome passenger complex (CPC) must relocate from anaphase chromosomes to the cell equator for successful cytokinesis. Although this landmark event requires the mitotic kinesin MKlp2, the spatiotemporal mechanistic basis remains elusive. Here, we show that phosphoregulation of MKlp2 by
Amitava Mukherjee et al.
PLoS pathogens, 7(3), e1001311-e1001311 (2011-03-26)
The host innate immune response to viral infections often involves the activation of parallel pattern recognition receptor (PRR) pathways that converge on the induction of type I interferons (IFNs). Several viruses have evolved sophisticated mechanisms to attenuate antiviral host signaling
Severa Bunda et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(36), E3785-E3794 (2014-08-27)
Mutations in Ras GTPase and various other components of the Ras signaling pathways are among the most common genetic alterations in human cancers and also have been identified in several familial developmental syndromes. Over the past few decades it has
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