OE19 Cell Line human

96071721, human esophagus, Epithelial

• Sinónimos: JROECL19, JROECL19 Cells, OE-19 Cells, OEC19 Cells
• UNSPSC Code: 41106514

Propiedades

• product name: OE19 Cell Line human, 96071721
• biological source: human esophagus
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Epithelial
• products: Not specified
• receptors: MHC class I
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian oesophageal carcinoma

Cell Line Description

The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

OE19 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study microRNA-330-5p as a modulator of neoadjuvant chemoradiotherapy sensitivity.

Study of oesophageal cancer

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17

Culture Medium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8, i.e., seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.

Other Notes

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