C8482
Protein Kinase A catalytic subunit human
>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Sinónimos:
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
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About This Item
Productos recomendados
recombinante
expressed in E. coli
Nivel de calidad
Ensayo
>90% (SDS-PAGE)
Formulario
buffered aqueous glycerol solution
actividad específica
>1000 units/mg protein
mol peso
43.5 kDa
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... PRKACA(5566)
Acciones bioquímicas o fisiológicas
Phosphorylates a spectrum of substrates, and regulates many cellular processes.
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
Forma física
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
Nota de análisis
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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