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C5467

SAFC

CHO Medium

Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fabricación farmacéutica

Sinónimos:

CHO Medium

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About This Item

Número MDL:
MFCD01634638
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

500

descripción

for research or for further manufacturing use

esterilidad

sterile-filtered

Formulario

liquid

calidad

Drug/Device Master File available

técnicas

cell culture | mammalian: suitable

componentes

HEPES: yes
NaHCO3: yes
phenol red: no
L-glutamine: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Categorías relacionadas

Aplicación

Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Características y beneficios

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 106 cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

Otras notas

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

Reconstitución

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Información legal

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000288080
RNBL8517
RNBL3238
RNBK8042
SLCP5281

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Artículos

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

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In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

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