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C4585

Sigma-Aldrich

Anti-β-Tubulin−Cy3 antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-β-Tubulin

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

CY3 conjugate

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

TUB 2.1, monoclonal

Formulario

buffered aqueous solution

mol peso

antigen 55 kDa

reactividad de especies

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

condiciones de almacenamiento

protect from light

técnicas

direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells

isotipo

IgG1

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. β-tubulin structure is characterized with the core of two β-sheets enclosed by α-helices.

Especificidad

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Inmunógeno

tubulin from rat brain.

Aplicación

Monoclonal Anti-β-Tubulin-Cy3 antibody produced in mouse has been used in:
  • immunohistochemical assays
  • immunofluorescence
  • western blotting
  • immunocytochemistry
  • immunoblotting
  • immunostaining

Acciones bioquímicas o fisiológicas

Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Información legal

Cy is distributed under license from Amersham Biosciences Limited.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Liang Qiang et al.
Molecular biology of the cell, 21(2), 334-344 (2009-11-27)
The formation of interstitial axonal branches involves the severing of microtubules at sites where new branches form. Here we wished to ascertain whether basic fibroblast growth factor (bFGF) enhances axonal branching through alterations in proteins involved in the severing of
Hong-Ryul Jung et al.
PloS one, 9(3), e91926-e91926 (2014-03-19)
Similar to stem cells, naïve T cells undergo asymmetric division following activation. While asymmetric division of T cells has been shown to be an important mechanism for the generation of lymphocyte fate diversity during immune responses, key factors that influence
Beta-and gamma-cytoplasmic actins are required for meiosis in mouse oocytes
Brockmann C, et al.
Biology of Reproduction, 85(5), 1025-1039 (2011)
M Janmaleki et al.
Scientific reports, 6, 32418-32418 (2016-09-02)
This study focused on the effects of simulated microgravity (s-μg) on mechanical properties, major cytoskeleton biopolymers, and morphology of endothelial cells (ECs). The structural and functional integrity of ECs are vital to regulate vascular homeostasis and prevent atherosclerosis. Furthermore, these
Comprehensive genotype-phenotype correlation in lissencephaly
Tan AP, et al.
Quantitative imaging in medicine and surgery, 8(7), 673-673 (2018)

Artículos

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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