A7310
Anti-Water Channel Aquaporin 2 antibody produced in rabbit
IgG fraction of antiserum, lyophilized powder
Sinónimos:
Anti-Aquaporin 2, Aquaporin 2 Detection, Rabbit Anti-Aquaporin 2
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About This Item
Productos recomendados
origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
IgG fraction of antiserum
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
lyophilized powder
reactividad de especies
rat, human
técnicas
immunohistochemistry: suitable
western blot (chemiluminescent): 1:200-1:1000
Nº de acceso UniProt
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... AQP2(359)
mouse ... Aqp2(11827)
rat ... Aqp2(25386)
Categorías relacionadas
Descripción general
Aquaporin 2 (AQP2) exists as a tetramer with C terminus showing multiple conformations and binds two cadmium ions. AQP2 gene is mapped to human chromosome 12q13.12. It is associated with intracellular vesicles.
Aquaporin 2 (AQP2) is a water channel protein that regulates water balance in renal connecting tubules and collecting ducts. AQP2 is activated by anti-diuretic hormone and subsequently facilitates water inflow in renal cells. This protein also interacts with integrin to modulate epithelial cell morphogenesis and cell movement . Anti-Water Channel Aquaporin 2 antibody recognizes aquaporin 2 from rat by immunoblotting. The antibody also reacts with mouse and human AQP2.
Inmunógeno
synthetic peptide corresponding to amino acids 254-271 of rat or mouse aquaporin 2 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde. The epitope is identical in rat and mouse and highly conserved in human.
Aplicación
Anti-Water Channel Aquaporin 2 antibody is also suitable for use in chemiluminescent western blot (1:200-1:1000), and immunoblot.
Anti-Water Channel Aquaporin 2 antibody produced in rabbit has been used in:
- immunostaining
- immunohistochemistry
- immunofluorescence
- immunoblotting
Immunohistochemistry was performed on 10% formalin-fixed parraffin embedded rat kidney sections using rabbit anti-AQP2 at a concentration of 5μg/ml. Use of anti-AQP2 allowed visualization of the medullary thick ascending limb and collecting ducts.
Acciones bioquímicas o fisiológicas
Aquaporin (AQP2) N-terminal region plays a key role in trafficking. The C-terminal phosphorylation is crucial for the water permeability function of the apical membrane. Also, ubiquitination at lysine 270 is needed for AQP2 sorting and degradation in lysosomes or elimination through exosomes. Mutation in AQP2 gene is implicated in congenital nephrogenic diabetes insipidus (NDI), a water balance disorder. The functional dysregulation of AQP2 is also correlated to preeclampsia, congestive heart failure and liver cirrhosis.
Forma física
Lyophilized from a solution containing phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide.
Nota de preparación
Isolated on immobilized Protein A
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Código de clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Alexandra Rieger et al.
PloS one, 11(7), e0158977-e0158977 (2016-07-16)
During nephrogenesis, POU domain class 3 transcription factor 3 (POU3F3 aka BRN1) is critically involved in development of distinct nephron segments, including the thick ascending limb of the loop of Henle (TAL). Deficiency of POU3F3 in knock-out mice leads to
Kuniaki Takata et al.
Histochemistry and cell biology, 130(2), 197-209 (2008-06-21)
Aquaporins (AQPs) are membrane proteins serving in the transfer of water and small solutes across cellular membranes. AQPs play a variety of roles in the body such as urine formation, prevention from dehydration in covering epithelia, water handling in the
Novel AVPR2 variant in a male infant with nephrogenic diabetes insipidus who showed delayed head control
Hasegawa K, et al.
Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology, 28(4), 155-158 (2019)
Khalil El Karoui et al.
Nature communications, 7, 10330-10330 (2016-01-21)
In chronic kidney disease (CKD), proteinuria results in severe tubulointerstitial lesions, which ultimately lead to end-stage renal disease. Here we identify 4-phenylbutyric acid (PBA), a chemical chaperone already used in humans, as a novel therapeutic strategy capable to counteract the
Jean-Charles Gautier et al.
Toxicologic pathology, 38(6), 943-956 (2010-08-19)
Cisplatin is an anticancer agent that induces renal proximal tubule lesions in many species. Studies were conducted in Sprague-Dawley and Han-Wistar rats to evaluate the utility of novel preclinical biomarkers of nephrotoxicity for renal lesions caused by this compound. Groups
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