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76245

Sigma-Aldrich

Atto 655 NHS ester

BioReagent, suitable for fluorescence

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About This Item

MDL number:
MFCD03456137
UNSPSC Code:
12352108
PubChem Substance ID:
93577785
NACRES:
NA.32

product line

BioReagent

assay

≥90.0% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

extent of labeling

≥90.0%

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 650.0-656.0 nm Amax

suitability

suitable for coupling to amines
suitable for fluorescence

storage temp.

−20°C

InChI

1S/C31H36N4O8S/c1-4-33-11-5-7-19-13-22-26(15-24(19)33)42-27-16-25-21(14-23(27)32-22)20(18-44(39,40)41)17-31(2,3)34(25)12-6-8-30(38)43-35-28(36)9-10-29(35)37/h13-16,20H,4-12,17-18H2,1-3H3

InChI key

SPDKWNQUMMQOKX-UHFFFAOYSA-N

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Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Gerald Giller et al.
Nucleic acids research, 31(10), 2630-2635 (2003-05-09)
Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2'-deoxyribonucleoside-5'-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5'-triphosphates of all four naturally occurring nucleobases were used as precursors. Commercially available dyes were
Taurai Tasara et al.
Nucleic acids research, 31(10), 2636-2646 (2003-05-09)
The modification of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here
Ye Chen et al.
Biomedical optics express, 10(3), 1257-1272 (2019-03-21)
Open-top light-sheet microscopy is a technique that can potentially enable rapid ex vivo inspection of large tissue surfaces and volumes. Here, we have optimized an open-top light-sheet (OTLS) microscope and image-processing workflow for the comprehensive examination of surgical margin surfaces
Highly sensitive protease assay using fluorescence quenching of peptide probes based on photoinduced electron transfer.
Nicole Marmé et al.
Angewandte Chemie (International ed. in English), 43(29), 3798-3801 (2004-07-20)
Nicole Marmé et al.
Bioconjugate chemistry, 14(6), 1133-1139 (2003-11-20)
Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655
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