59747

Lactic Dehydrogenase, recombinant

≥90 U/mg

• Sinónimos: (S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase
• Número de CAS: 9001-60-9
• Comisión internacional de enzimas: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL number: MFCD00131466
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• recombinant: expressed in E. coli
• form: powder
• specific activity: ≥90 U/mg
• storage temp.: −20°C

Descripción

General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

Application

Lactic Dehydrogenase, recombinant from E. coli has been used:

• in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
• to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
• in in vitro DltC D-alanylation assay

Biochem/physiol Actions

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

Unit Definition

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)