The product is supplied sterile and should be reconstituted with 5ml of sterile 0.01M HCl. We would recommend filter sterilising the 0.01M HCl without collagen using a Millex syringe filter such as SLLG025SS (solvent resistant PTFE) or as it is only a weak acid solution then you could use cheaper SLGVR33RS (PVDF) sterile filters. It is not advisable to filter the collagen once in solution.
5006
Bovine Collagen Type I & III
from bovine hide, powder, suitable for cell culture, PureCol™
Sinónimos:
Collagen Type I, PureCol Collagen
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About This Item
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Nombre del producto
PureCol™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture
origen biológico
bovine
Nivel de calidad
esterilidad
sterile-filtered
Ensayo
99.9%
Formulario
lyophilized
envase
pkg of 15 mg
técnicas
cell culture | mammalian: suitable
Especificidad de unión
Peptide Source: Elastin
Peptide Source: Fibrinogen
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.
It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.
Aplicación
PureCol™ has been used:
- in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids[1]
- to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)[2]
- to coat culture plates for collagen degradation assay with breast cancer cell lines[3]
Nota de preparación
This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.
Información legal
Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
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How can this product be sterilised after reconstitution to 3 mg/ml?
1 answer-
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Howlong is this product stable after reconstructiond and under which conditions should this stock solution be stored?
1 answer-
The long term stability of this product in solution has not been determined. However, historical data suggests that solutions stored protected from light, in amber glass or wrapped in foil, are stable at least 30 days when stored refrigerated at 2 - 8°C.
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