47608

Formaldehído solution

for molecular biology, BioReagent, ≥36.0% in H₂O (T)

• Sinónimos: Formalina
• Fórmula lineal: HCHO
• Número de CAS: 50-00-0
• Peso molecular: 30.03
• Beilstein/REAXYS Number: 1209228
• MDL number: MFCD00003274
• UNSPSC Code: 12352114
• PubChem Substance ID: 329757328
• NACRES: NA.52

Propiedades

• grade: for molecular biology
• Quality Level: 100
• vapor density: 1.03 (vs air)
• vapor pressure: 52 mmHg ( 37 °C)
• product line: BioReagent
• form: liquid
• autoignition temp.: 572 °F
• contains: ~10% methanol as stabilizer
• concentration: ≥36.0% in H₂O (T); 36.0-38.0% (aldehyde method)
• technique(s): electrophoresis: suitable
• impurities: ≤0.03% free acid (as HCOOH)
• ign. residue: ≤0.05% (as SO₄)
• pH: 2.8-4.0
• density: 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• cation traces: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/mL
• λ: 2 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.01; λ: 280 nm A_max: 0.01
• suitability: in accordance for electrophoresis of RNA in agarose gels
• storage temp.: room temp
• SMILES string: [H]C([H])=O
• InChI: 1S/CH2O/c1-2/h1H2
• InChI key: WSFSSNUMVMOOMR-UHFFFAOYSA-N

Descripción

General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Application

Formaldehyde solution is suitable for:

• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Formaldehydesolution has been used:

• in the fixation of human spermatogonial stem cell transplantation (SSCT).
• to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
• in fixing zebra fish larvae for neutrophil staining.

Caution

may contain precipitate (paraformaldehyde)

Principle

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Información de seguridad

• pictograms: GHS02,GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H226,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• pcodes: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• target_organs: Eyes,Central nervous system, Respiratory system
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 3
• flash_point_f: 133.0 °F - closed cup
• flash_point_c: 56.11 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter