B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• Sinónimos: 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• Fórmula empírica (notación de Hill): C₃₃H₃₂N₄O₈S
• Peso molecular: 644.69
• Número MDL: MFCD09264223
• Código UNSPSC: 12171500
• eCl@ss: 34058011
• ID de la sustancia en PubChem: 329773319
• NACRES: NA.47

Propiedades

• Nombre del producto: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Nivel de calidad: 100; 200
• Ensayo: ≥95.0% (HPLC)
• Formulario: powder
• mol peso: 644.71
• técnicas: titration: suitable
• color: light orange to very dark orange; light yellow to very dark yellow
• ε (coeficiente de extinción): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56
• InChI: 1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30
• Clave InChI: UROXVNHIYQXTRA-LFERIPGTSA-N

Descripción

Aplicación

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Acciones bioquímicas o fisiológicas

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)