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11333089001

Roche

Anti-Digoxigenin

from sheep

Sinónimos:

anti-digoxigenin, digoxigenin

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About This Item

Código UNSPSC:
12352203
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

sheep

Nivel de calidad

100

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

lyophilized

envase

pkg of 200 μg

fabricante / nombre comercial

Roche

isotipo

IgG

temp. de almacenamiento

2-8°C

Descripción general

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.[1]
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.[2]

Especificidad

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Aplicación

Anti-Digoxigenin has been used in DNA tethering.[3] It has been used to attach DNA molecule to the glass surface of the flow cell.[4]
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry[5]
  • In situ hybridization[6]
  • Western blot

Acciones bioquímicas o fisiológicas

In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.[7]

Nota de preparación

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

Reconstitución

Add 1 ml PBS to a final concentration of 200 μg/ml.

Nota de análisis

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Otras notas

For life science research only. Not for use in diagnostic procedures.

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Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

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Certificados de análisis (COA)

Lot/Batch Number
67442300
60789300
49170100
34244900
66890900

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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+?ATPase by N?Hydroxysuccinimidyl Derivatives of Digoxigenin.
Antolovic R, et al.
European Journal of Biochemistry, 227(1?2), 61-67 (1995)
Juliette Salvaing et al.
PloS one, 7(5), e38156-e38156 (2012-06-14)
In the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the mechanisms responsible for it remain elusive.
Pengyu Hao et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(30), 17775-17784 (2020-07-17)
DNA mismatch repair (MMR), the guardian of the genome, commences when MutS identifies a mismatch and recruits MutL to nick the error-containing strand, allowing excision and DNA resynthesis. Dominant MMR models posit that after mismatch recognition, ATP converts MutS to
Chan Chen et al.
Aging, 12(14), 14490-14505 (2020-07-22)
Cardiac arrest (CA) is the leading cause of death around the world. Survivors after CA and cardiopulmonary resuscitation (CPR) develop moderate to severe cognitive impairment up to 60% at 3 months. Accumulating evidence demonstrated that long non-coding RNAs (lncRNAs) played
Electron paramagnetic resonance of a single NV nanodiamond attached to an individual biomolecule.
Teeling-Smith R M, et al.
Biophysical Journal, 110(9), 2044-2052 (2016)
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