411492
Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)
liquid, clone HP6025, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
secondary antibodies
clone
HP6025, monoclonal
form
liquid
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody. Recognizes human IgG4 Fc fragment.
This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.
Application
Enzyme Immunoassay (10 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Other Notes
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤0.04%
Human IgG3: ≤0.02%
Human IgG4: 100%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: 100%
Human IgA: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤0.04%
Human IgG3: ≤0.02%
Human IgG4: 100%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: 100%
Human IgA: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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D C Houpt et al.
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Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
R G Keller et al.
Infection and immunity, 62(1), 215-220 (1994-01-01)
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