SAE0196

Achromopeptidase from bacteria

free of DNA contaminants, suitable for Microbiome research

• Synonyme(s) : Bacterial Protease
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥1000 U/mg
• Caractéristiques: DNA free
• Technique(s): DNA extraction: suitable
• Adéquation: suitable for cell lysis
• Application(s): cell analysis
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.

The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Actions biochimiques/physiologiques

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.

Caractéristiques et avantages

Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms

Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods

Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA

Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies

Définition de l'unité

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable