Solutions of lyticase can be prepared in DNA-free water. One publication reports the preparation of stock solutions of lyticase at 5 mg/mL in 1 M sorbitol with 0.1 M EDTA, pH 8.0, with storage of these stock solutions at –20 C in frozen aliquots. However, this method has not been tested by us. The lyticase may also be dissolved in 50 mM potassium phosphate buffer, pH 7.5, with 50% glycerol and stored at -20 deg. Solutions of Lyticase are not considered to be stable, and it is recommended that solutions be prepared fresh and not stored as stock solutions. We do not have information on the long-term stability if stored at -20 C. We would advise using it within a period of 1 month if stored at -20 C in aliquots.
SAE0098
Lyticase from Arthrobacter luteus
free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder
Synonyme(s) :
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
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About This Item
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Produits recommandés
Source biologique
bacterial (Arthrobacter luteus)
Forme
lyophilized powder
Activité spécifique
≥2000 units/mg protein
Caractéristiques
DNA free
Technique(s)
cell based assay: suitable
Adéquation
suitable for cell lysis
Application(s)
diagnostic assay manufacturing
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Actions biochimiques/physiologiques
La lyticase hydrolyse le poly-β(1→3)-glucose, notamment le glucane de la paroi cellulaire des levures.
Définition de l'unité
Une unité produit une ΔA800 de 0,001 par minute à pH 7,5 et 25 °C, en utilisant comme substrat une suspension de levure dans un mélange réactionnel de 3 ml.
Autres remarques
Pour en savoir plus sur les enzymes utilisées dans l′analyse des glucides complexes, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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What recommendations are available for the reconstitution of Lyticase? Should it be prepared in water or a buffer? Can you provide general information as well as specific information for product SAE0098?
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