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R3779

Sigma-Aldrich

Anti-RbAp48/RbAp46 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-Retinoblastoma-binding protein

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About This Item

Numéro MDL:
MFCD02686588
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 46 kDa (RbAp46)
antigen 48 kDa (RbAp48)

Espèces réactives

human

Technique(s)

indirect immunofluorescence: 1:500-1:1,000 using human HeLa cells fixed with paraformaldehyde-Triton
microarray: suitable
western blot: 1:2,000-1:4,000 using MCF7 nuclear extracts

Numéro d'accès UniProt

Q16576

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RBBP4(5928) , RBBP7(5931)
mouse ... Rbbp4(19646) , Rbbp7(245688)
rat ... Rbbp4(313048) , Rbbp7(83712)

Description générale

pRB-associated proteins p48 (RbAp48) is a 425 amino acids WD-domain protein isolated as a RB1 (retinoblastoma binding protein). RbAp46 (also known as RBBP7- retinoblastoma-binding protein 7) has 89% amino acid identity with RbAp48.

Immunogène

synthetic peptide corresponding to amino acids 413-425 of human RbAp48, conjugated to KLH via an N-terminal added lysine residue. The immunizing peptide is conserved in human, mouse, and rat.

Application

Anti-RbAp48/RbAp46 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Actions biochimiques/physiologiques

RbAp46 and RbAp48 are two histone tail-targeting proteins, that are found in association with chromatin remodeling complexes Sin3A/ histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex). Transcriptional repressors exert their effects by recruitment of the Sin3A/HDAC corepressor complex, which contains a module composed of Sin3A, HDAC1, HDAC2, RbAp46, RbAp48, SAP30, and others. In the NuRD complex, HDAC1, HDAC2, RbAp48, and RbAp46 associate with MTA2 (metastasis associated 1 family member 2), MBD3 (methyl-CpG binding domain protein), MAT1L1, MBD3L1 (methyl-CpG binding domain protein 3 like 1), CHD3, and CHD4 to form the nucleosomal remodeling and deacetylation (NuRD) complex.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Identification and Characterization of Three New Components of the mSin3A Corepressor Complex
Fleischer TC, et al.
Molecular and Cellular Biology, 23, 3456-3467 (2003)
The strategy of fusion genes construction determines efficient expression of introduced transcription factors.
Adamus T, et al.
Acta Biochimica Polonica, 61(4) (2014)
Salomé Manska et al.
Virology, 566, 26-41 (2021-12-04)
Upon entry of Human cytomegalovirus (HCMV) into the host cell, the viral genome is transported to the nucleus where it serves as a template for transcription and genome replication. Production of new viral genomes is a coordinated effort between viral
Seung-Gi Jin et al.
The Journal of biological chemistry, 280(13), 12700-12709 (2005-02-11)
MBD2 and MBD3 are two proteins that contain methyl-CpG binding domains and have a transcriptional repression function. Both proteins are components of a large CpG-methylated DNA binding complex named MeCP1, which consists of the nucleosome remodeling and histone deacetylase complex
Yongqiang Hou et al.
Science advances, 6(16), eaaz0356-eaaz0356 (2020-06-05)
TUDOR domain-containing proteins (TDRDs) are chiefly responsible for recognizing methyl-lysine/arginine residue. However, how TDRD dysregulation contributes to breast tumorigenesis is poorly understood. Here, we report that TUDOR domain-containing PHF20L1 as a H3K27me2 reader exerts transcriptional repression by recruiting polycomb repressive
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