Accéder au contenu
Merck
Toutes les photos(17)

Documents

ZMS1070

Sigma-Aldrich

Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1H6, recombinant monoclonal

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Espèces réactives

human, mouse

Réactivité de l'espèce (prédite par homologie)

all

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Isotype

IgG2bκ

Séquence de l'épitope

Unknown

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 1H6 is a ZooMAb® mouse recombinant monoclonal antibody that binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity. It displays no detectable binding to a RNA hairpin, single-stranded DNA or double-stranded DNA.

Immunogène

KLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in mouse kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected DNA G-quadruplex (G4) in mouse kidney tissue sections.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DNA G-quadruplex (G4) in HeLa, A431, and NIH 3T3 cells.

Flow Cytometry Analysis: 1 μg from a representative lot detected DNA G-quadruplex (G4) in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-DNA G-quadruplex (G4), clone 1H6 ZooMAb®, Cat. No. ZMS1070, is a recombinant Mouse monoclonal antibody that targets DNA G-quadruplex (G4) and is tested for use in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry (Paraffin).

Description de la cible

DNA G-quadruplex structures are associated with a number of important aspects of genome function, which include transcription, recombination and replication. Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4). Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an appealing topic in molecular biomedical research. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique