Both package sizes are bottles that are sealed in a polyamide bag. This bag is placed, with additional absorbent material and padding, in a metal can with a pull tap. The final packaged item is placed in the shipping box that is most compatible with the order. This product is shipped at ambient temperature.
P4557
Phénol solution
BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology
Synonyme(s) :
Tris-equilabrated Phenol
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About This Item
Formule empirique (notation de Hill):
C6H6O
Numéro CAS:
Poids moléculaire :
94.11
Beilstein:
6428463
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352104
ID de substance PubChem :
Nomenclature NACRES :
NA.52
Qualité:
for molecular biology
Essai:
≥99.0% (GC)
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Gamme de produits
BioReagent
Essai
≥99.0% (GC)
Forme
liquid
pH
7.7-8.1 (25 °C)
Température de stockage
2-8°C
Chaîne SMILES
Oc1ccccc1
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
Clé InChI
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
Informations sur le gène
human ... GABRA1(2554)
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Description générale
A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.[1]
Application
Phenol solution has been used:
- as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
- for extraction of DNA from Arabidopsis thaliana Rosette leaves
- in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
- as a component of extraction buffer to extract RNA from fruit pulp
- for the extraction of protein from roots[2]
- for the isolation of fecal DNA[3]
- for the extraction of caged RNA[4]
Actions biochimiques/physiologiques
The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.[5]
Composants
- 1 bottle Tris-equilabrated Phenol (product number P4557)
- Equilibration Buffer (product B5658)
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Organes cibles
Nervous system,Kidney,Liver,Skin
Code de la classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Les clients ont également consulté
Fecal Microbiome and Food Allergy in Pediatric Atopic Dermatitis: A Cross-Sectional Pilot Study
Fieten KB, et al.
International Archives of Allergy and Immunology, 175(1-2), 77-84 (2018)
PHENOLS
Smith PWG and Tatchell AR
Aromatic Chemistry, 79-104 (1969)
Nucleic Acid Extraction Techniques
Yi-Wei Tang and Charles W. Stratton
Journal of Separation Science (2012)
Protocol Optimization of Proteomic Analysis of Korean Ginseng (Panax ginseng Meyer)
Braccia C, et al.
Journal of Separation Science, 8(4), 53-53 (2021)
Tim J Bull et al.
Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'
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What is the export packaging? Is it in thermocol/Foam packaging? Can you share the packaging Image.
1 answer-
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What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
1 answer-
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
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I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
1 answer-
The top phase is Tris buffer, so use the lower/bottom phase.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molarity of Phenol solution, Product P4557?
1 answer-
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
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What is the final pH of Phenol solution, Product P4557, after adding the buffer?
1 answer-
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
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