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L7920

Sigma-Aldrich

β-Lactamase Blend

recombinant, expressed in E. coli

Synonyme(s) :

Carbapenemase, Cephalosprinase

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About This Item

Numéro de classification (Commission des enzymes):
3.5.2.6 (BRENDA, IUBMB)
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Stérilité

γ-irradiated

Forme

lyophilized powder

Température de stockage

2-8°C

Description générale

No detectable growth in tryptone soy broth at 30-35 °C for 14 days

Actions biochimiques/physiologiques

β-Lactamases exhibit high carbapenemase activity. β-lactamases can destroy the β-lactam ring.[1] True carbapenemases has the ability to hydrolyse several β-lactam ring containing carbapenems like meropenem, doripenem, imipenem and ertapenem.[2]

Composants

600-1500 IU beta-lactamase I per vial
60-150 IU beta-lactamase II per vial

Définition de l'unité

One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in phosphate buffer, pH 7.0 at 25°C.

Forme physique

Lyophilized powder containing sodium chloride, potassium phosphate, dipotassium phosphate and sorbitol.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCM4634
SLCQ2635
SLCM5001
SLCP4519
SLCM1748

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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Lisa M Miller et al.
ACS applied materials & interfaces, 11(36), 32599-32604 (2019-08-27)
Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial
Mechanisms and Detection of Antimicrobial Resistance
Principles and Practice of Pediatric Infectious Diseases (Fourth Edition), 1467-1478 (2017)
Identification and screening of carbapenemase-producing Enterobacteriaceae
Nordmann P, et al.
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(5), 432-438 (2012)
Matthew D Simmons et al.
Antibiotics (Basel, Switzerland), 9(10) (2020-10-03)
The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI's) occurring in the general population. Most technologies developed for the detection of DRI's typically focus on phenotyping or genotyping bacterial resistance rather than
Joseph E Rubin et al.
Veterinary microbiology, 170(1-2), 10-18 (2014-03-01)
Organisms in the family Enterobactericeae including, Escherichia coli, commonly cause community and hospital-associated infections in both humans and companion animals. The increasing prevalence of infections with organisms producing broad spectrum β-lactamses such as the ESBLs (particularly the CTX-M type), AmpC
Afficher tous les articles scientifiques apparentés

Questions

1–2 of 2 Questions  

  1. Dear Mr/Ms, I ordered β-Lactamase Blend L7920 and I am interested in knowing the concentration in micrograms per mL. (Micrograms per mL=IU/mL/min×Conversion Factor). Can you please tell me what is the conversion factor? Regards, Glorjen

    1 answer

    1. This product is provided in lyophilized solid form with the unit activity reported in lot specific Certificate of Analysis.

      Using a representative lot, SLCQ2635 as an example, one vial contains 1289 units. The activity can be converted to mass in milligrams using the batch specific activity value of 124 units/mg.

      Thus, a vial from batch (SLCQ2635) contains 10.39 mg of enzyme; 1289 units x (1 mg / 124 unit) = 10.39 mg.

      The volume in mL would be dependent on the volume of solvent added. For example, adding 1 mL of solvent to a vial from this batch, would result in a concentration of 10.39 mg/mL.

      Helpful?

  2. HOW TO DISSOLVE THE VIAL

    1 answer

    1. This product has been sterilized by gamma-irradiaton and can be reconstituted with in 10 mL of deionized purified water, then filter sterilzed through a 0.2 micron low protein binding membrane. If being used for the preparation of agar plates, it should be added to the medium at a temperature of less than 55 deg C. After reconstitution, the solution is stable for 2 months at 2-8 deg C."

      Helpful?

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