P4524

β-Lactamase from Enterobacter cloacae

Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)

• Synonyme(s) : β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
• Numéro CAS: 9073-60-3
• Numéro de classification (Commission des enzymes): 3.5.2.6 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131819
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial (Enterobacter cloacae)
• Niveau de qualité: 200
• Type: Type IV
• Forme: lyophilized powder
• Activité spécifique: 0.2-0.6 units/mg protein (using benzylpenicillin)
• Poids mol.: 29 kDa
• Composition: Protein, ~60%
• Technique(s): enzyme immunoassay: suitable
• Adéquation: suitable for enzyme test
• Application(s): pharmaceutical
• Température de stockage: 2-8°C

Description

Description générale

β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria.

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder.

β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures.

Actions biochimiques/physiologiques

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Définition de l'unité

1 U hydrolysiert 1.0 μmol des angegebenen Substrats pro Minute bei pH 7.0 und 25 °C. Wird als Benzylpenicillin Einheiten verkauft. Diese International Einheit (unter Verwendung von Benzylpenicillin als Substrat) entspricht ca. 600 Levy oder 75 Pollock Einheiten.

Forme physique

Lyophilized powder containing sodium phosphate buffer salts

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves