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L2654

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O26:B6

γ-irradiated, BioXtra, suitable for cell culture

Synonyme(s) :

LPS

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About This Item

Numéro CAS:
93572-42-0
Numéro CE :
297-473-0
Numéro MDL:
MFCD00164401
Code UNSPSC :
12352211
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

Escherichia coli (O26:B6)

Niveau de qualité

300

Stérilité

γ-irradiated

Gamme de produits

BioXtra

Forme

lyophilized powder

Produit purifié par

gel-filtration chromatography

Technique(s)

cell culture | mammalian: suitable

Impuretés

<5% Protein (Lowry)

Solubilité

H2O: 5 mg/mL, slightly hazy

Température de stockage

2-8°C

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Catégories apparentées

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli 026:B6 was used to elicit the secretion of cytokines by human PBMC[1], murine bone marrow dendritic cells[2] and rat astrocytes.[3]

Actions biochimiques/physiologiques

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pictogrammes

Skull and crossbones
GHS06

Mention d'avertissement

Danger

Mentions de danger

H300

Classification des risques

Acute Tox. 2 Oral

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000381521
0000381521
0000242756
108M4192V
12180308

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ø Salvesen et al.
Journal of neuroinflammation, 14(1), 106-106 (2017-05-24)
The cellular prion protein (PrP In order to explore putative roles for PrP All LPS-treated goats displayed clinical signs of sickness behavior, which were of significantly (p < 0.01) longer duration in animals without PrP Our data suggest that PrP
David Gozal et al.
Sleep, 33(3), 319-325 (2010-03-27)
Sleep disordered breathing in children is associated with severity-dependent increases in excessive daytime sleepiness (EDS). TNF-alpha is an inflammatory cytokine that has been implicated in EDS. Since, at any given level of apnea-hypopnea index, there is significant variability in EDS
S Ménard et al.
Gut, 53(6), 821-828 (2004-05-13)
Probiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor alpha (TNF-alpha) release and the mechanisms involved.
Patricia Cassina et al.
Journal of neuroscience research, 67(1), 21-29 (2001-12-26)
Oxidative stress mediated by nitric oxide (NO) and its toxic metabolite peroxynitrite has previously been associated with motor neuron degeneration in amyotrophic lateral sclerosis (ALS). Degenerating spinal motor neurons in familial and sporadic ALS are typically surrounded by reactive astrocytes
Peta J O'Connell et al.
Blood, 107(3), 1010-1017 (2005-10-15)
Adaptive immunity is triggered at the immune synapse, where peptide-major histocompatibility complexes and costimulatory molecules expressed by dendritic cells (DCs) are physically presented to T cells. Here we describe transmission of the inflammatory monoamine serotonin (5-hydroxytryptamine [5-HT]) between these cells.
Afficher tous les articles scientifiques apparentés

Articles

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Questions

  1. LPS（Product number:L 2654）はTLR2に結合しますか？

    1 answer

    1. Lipopolysaccarides from Escherichia coli O26:B6 are expected to only interact with Toll-like receptor 4 (TLR4). Similar lipopolysaccharides from Porphyromonas gingivalis have demonstrated the ability to bind with both TLR2 and TLR4. This observation is described more fully in the peer-reviewed publication, "Effects of serotype and species dependency of bacterial lipopolysaccharides in human melanocytes from lightly and darkly-pigmented skin," and in its associated citations. A link to this article from PubMed Central can be found here:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074968/

      Please see the link below to review product SMB00610, LPS from Porphyromonas gingivalis"
      https://www.sigmaaldrich.com/product/sigma/smb00610

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