This product is tested for solubility at 5 mg/mL in water. Aqueous buffers such as DPBS are expected to be suitable as well. Pease see the link below to review a reference that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685187/
L2637
Lipopolysaccharides from Escherichia coli O55:B5
purified by gel-filtration chromatography
Synonyme(s) :
LPS
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
Escherichia coli (O55:B5)
Niveau de qualité
Forme
lyophilized powder
Produit purifié par
gel-filtration chromatography
Impuretés
<3% Protein
Couleur
white to faint gray
Solubilité
water: soluble
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
This product is extracted from E. coli serotype O55:B5 and purified by gel filtration. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.
Application
Les lipopolysaccharides (LPS) sont des constituants caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné via le récepteur TLR4 (Toll-Like Receptor 4), un membre de la famille des récepteurs de type Toll, qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).
Actions biochimiques/physiologiques
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Notes préparatoires
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Autres remarques
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 2 Oral
Code de la classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Articles
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Contenu apparenté
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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