KYSE-410

94072023, human esophagus, Epitheloid

• Synonyme(s) : KYSE0410 Cells, KYSE410 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: KYSE-410, 94072023
• Source biologique: human esophagus
• Mode de croissance: Adherent
• Caryotype: Hypotetraploid with 4% polyploidy
• Morphologie: Epitheloid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human oesophageal squamous cell carcinoma

Description de la lignée cellulaire

Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18

Milieu de culture

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C. Doubling time ~45h

Autres remarques

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