JY human cell line

IHW Number: 9287, 94022533

• Synonyme(s) : JY-S
• Code UNSPSC : 12352207

Propriétés

• Source biologique: human blood
• Ethnicité: Amish
• Sexe: male
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblastoid
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Description générale

Consanguineous: No

Homozygous: No

Description de la lignée cellulaire

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profil d'ADN

A: 201
Cw: 702
B: 702
HLA DQA1: 3011
HLA DPB1: 201

Profil sérologique

HLA A: 2
HLA C: 7
HLA B: 7
Bw4/bw6: 6
HLA DR: 4 13
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3

Milieu de culture

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Procédure de repiquage

When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶ cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵ cells/ml. Maintain cultures between 3-9 x 10⁵ cells/ml for maximum growth; 5% CO₂; 37°C. Cells form floating aggregates of varying size.

Autres remarques

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Clause de non-responsabilité

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.