KELLY Cell Line human

92110411, human brain, Neuronal

• Synonyme(s) : NB19 Cells, NB19-RIKEN Cells
• Code UNSPSC : 41106514

Propriétés

• product name: KELLY Cell Line human, 92110411, neuroblastoma
• Source biologique: human brain
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Neuronal
• Produits: N-myc RNA or protein
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human neuroblastoma

Description de la lignée cellulaire

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Application

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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